Virginia Tech/Updates/Plasmid/Design

From 2007.igem.org

(Difference between revisions)
m (Virginia Tech/Plasmid moved to Virginia Tech/Updates/Plasmid)
m
Line 1: Line 1:
 +
<!--first table -->
 +
{| cellspacing="2px" border="2" style="padding: 0px; width: 750px; color: #8b0000; background-color: #8b0000;"
 +
<!-- first cell of the first table -->
 +
| style="padding: 5px; width: 100px; background-color: #8b0000; border: 2px solid #ff8c00;" |
 +
 +
<html><a href="https://2007.igem.org/Virginia_Tech"><img src="https://static.igem.org/mediawiki/2007/9/9f/VT_logo_sm.jpg" alt="Home"></a></html>
 +
<html><center><A href="https://2007.igem.org/Virginia_Tech" target=_top><h3><FONT color=#ff8c00>Home</FONT></h3></A></center></html>
 +
 +
<!-- second cell of the first table -->
 +
| style="padding: 5px; width: 100px; background-color: #8b0000; border: 2px solid #ff8c00;" |
 +
 +
<html><center><A href="https://2007.igem.org/Virginia_Tech/Project" target=_top><h3><FONT color=#ff8c00>Project</FONT></h3></A></center></html>
 +
 +
<!-- third cell of the first table -->
 +
| style="padding: 5px; width: 100px; background-color: #8b0000; border: 2px solid #ff8c00;" |
 +
 +
<html><center><A href="https://2007.igem.org/Virginia_Tech/Team" target=_top><h3><FONT color=#ff8c00>Team</FONT></h3></A></center></html>
 +
 +
<!-- fourth cell of the first table -->
 +
| style="padding: 5px; width: 100px; background-color: #8b0000; border: 2px solid #ff8c00;" |
 +
 +
<html><center><A href="https://2007.igem.org/Virginia_Tech/Photos" target=_top><h3><FONT color=#ff8c00>Photo Gallery</FONT></h3></A></center></html>
 +
 +
<!-- fifth cell of the first table -->
 +
| style="padding: 5px; width: 100px; background-color: #8b0000; border: 2px solid #ff8c00;" |
 +
 +
<html><center><A href="https://2007.igem.org/Virginia_Tech/JC" target=_top><h3><FONT color=#ff8c00>Journal Club</FONT></h3></A></center></html>
 +
 +
<!-- sixth cell of the first table -->
 +
| style="padding: 5px; width: 100px; background-color: #8b0000; border: 2px solid #ff8c00;" |
 +
 +
<html><center><A href="https://2007.igem.org/Virginia_Tech/Links" target=_top><h3><FONT color=#ff8c00>Links</FONT></h3></A></center></html>
 +
 +
<!-- seventh cell of the first table -->
 +
| style="padding: 5px; width: 100px; background-color: #8b0000; border: 2px solid #ff8c00;" |
 +
 +
<html><center><A href="mailto:igem@vt.edu" target=_top><h3><FONT color=#ff8c00>Contact
 +
</FONT></h3></A></center></html>
 +
 +
 +
|}
 +
 +
<!--second table -->
 +
{| cellspacing="2px" border="2" style="padding: 0px; width: 750px; color: #8b0000; background-color: #8b0000;"
 +
 +
<!-- first cell of the second table-->
 +
| style="padding: 5px; background-color: #000000; border: 2px solid #ff8c00;" |
 +
 +
<center><h1><font color="#ff8c00"> Reporter Plasmid Updates:
 +
</font></h1></center>
 +
<center><h3><font color="#8b0000"> VT iGEM Project 2007: Engineering an Epidemic</font></h3></center>
 +
 +
|}
 +
 +
<!--third table -->
 +
{| cellspacing="2px" border="2" style="padding: 0px; width: 750px; color: #8b0000; background-color: #8b0000;"
 +
 +
<!-- first cell of the third table-->
 +
| style="padding: 5px; background-color: #ffffff; border: 2px solid #ff8c00;" |
 +
 +
<font color="#000000">
 +
 +
<p><h3>Introduction: The Reporter Plasmid</h3>
 +
In order to collect data for our experiments, we needed a way to tell whether an infected cell was lysogenic or lytic. We decided that a good way to do this would be to use a reporter plasmid containing the lambda promoter and two fluorescent genes. The same chemicals that decided which pathway the cell entered would decide which fluorescent protein from our reporter plasmid to express.</p>
 +
 +
<p><h3>Updates</h3></p>
 +
 +
<p>'''Designing the Promoter Construct'''<br>
 +
''July 24, 2007''</p>
 +
<p>Click [[ here]] to read about how we designed the reporter plasmid's main feature, the promoter construct.</p>
 +
 +
 +
</font>
 +
|}
 +
 +
<!--fourth table -->
 +
{| cellspacing="2px" border="2" style="padding: 0px; width: 750px; color: #8b0000; background-color: #8b0000;"
 +
 +
<!-- first cell of the second table-->
 +
| style="padding: 5px; background-color: #000000; border: 2px solid #ff8c00;" |
 +
 +
 +
 +
<html><center><A href="https://2007.igem.org/Virginia_Tech/Updates" target=_top><h3><FONT color=#ff8c00>Return to Progress Updates</FONT></h3></A></center></html>
 +
 +
 +
 +
|}

Revision as of 17:05, 24 July 2007

Home

Home

Project

Team

Photo Gallery

Journal Club

Links

Contact


Reporter Plasmid Updates:

VT iGEM Project 2007: Engineering an Epidemic

Introduction: The Reporter Plasmid

In order to collect data for our experiments, we needed a way to tell whether an infected cell was lysogenic or lytic. We decided that a good way to do this would be to use a reporter plasmid containing the lambda promoter and two fluorescent genes. The same chemicals that decided which pathway the cell entered would decide which fluorescent protein from our reporter plasmid to express.

Updates

Designing the Promoter Construct
July 24, 2007

Click here to read about how we designed the reporter plasmid's main feature, the promoter construct.



Return to Progress Updates