Virginia Tech/phage

From 2007.igem.org

< Virginia Tech(Difference between revisions)
m
m
 
(3 intermediate revisions not shown)
Line 42: Line 42:
<img src="https://static.igem.org/mediawiki/2007/d/d5/Button_jc.JPG"/></a></html>[[Image:Dot1.JPG]]
<img src="https://static.igem.org/mediawiki/2007/d/d5/Button_jc.JPG"/></a></html>[[Image:Dot1.JPG]]
-
<!--sixth cell of second table: Links and Contact-->
+
<!--sixth cell of second table: Contributions and Contact-->
| style="padding: 0px; width=50px; background-color: #FFFFFF;" |
| style="padding: 0px; width=50px; background-color: #FFFFFF;" |
-
<html><a href="https://2007.igem.org/Virginia_Tech/Links">
+
<html><a href="https://2007.igem.org/Virginia_Tech/Contributions">
-
<img src="https://static.igem.org/mediawiki/2007/7/7e/Button_links.JPG"/></a></html><html><a href="mailto:igem@vt.edu"></a></html>
+
<img src="https://static.igem.org/mediawiki/2007/6/69/Button_contribute.JPG"/></a></html><html><a href="mailto:igem@vt.edu"></a></html>
|}<html></center></html>
|}<html></center></html>
Line 74: Line 74:
[[Image:Fl_phage.PNG|center|]]<html><center></html><h3>Bacteriophage λ</h3><html></center></html>
[[Image:Fl_phage.PNG|center|]]<html><center></html><h3>Bacteriophage λ</h3><html></center></html>
   
   
-
λ phage is the viral component in our biological system that we used to test our model. λ phage has been characterized and studied for decades. This is very important because we wanted to utilize a virus that was well known, thus allowing the team to understand λ. We also wanted to use a virus that was very stable and relatively easy to work with; λ phage again. The only problem with this was the fact that no one on the team had ever worked with viruses, let alone λ phage, before.   
+
λ phage is the viral component in our biological system that we used to test our model. We chose λ phage because it has been characterized and studied for decades. λ phage is also very stable and relatively easy to work with. We had a lot to learn since no one on the team had ever worked with λ phage before.   
-
           
+
 
-
Before we dove in and started working with the small amount of phage (WT and Fluorescent) made available to us, we decided that the best approach was to read as much as we could on the handling of phages. Thus, we spent the first week of the summer reading numerous protocols on how to multiply phage in plates and in liquid culture. We also had to learn how to work with the Fluorescent λ phage that was sent to us from the Laboratoire de Physique Statistique, Ecole Normale Supérieure, Paris, France. The phage was provided by Phillippe Thomen and is characterized in "Propagation of fluorescent viruses in growing plaques" by Alvarez LJ, Thomen P, Makushok T, Chatenay D.
+
At the beginning of the summer, we received fluorescent λ phage from the Laboratoire de Physique Statistique, Ecole Normale Supérieure, Paris, France. The phage was provided by Philippe Thomen and is characterized in <html><a href="https://static.igem.org/mediawiki/2007/3/32/Alvarez_2007.pdf">"Propagation of fluorescent viruses in growing plaques"</a></html> by Alvarez, L. J., P. Thomen, et al. Using this phage we were able to observe the build-up of viral particles in a lytic cell.

Latest revision as of 03:22, 27 October 2007

Title progress.JPG

Dot1.JPG

Dot1.JPG

Dot1.JPG

Dot1.JPG

Dot1.JPG

The Fluorescent Phage

Fl phage.PNG

Bacteriophage λ

λ phage is the viral component in our biological system that we used to test our model. We chose λ phage because it has been characterized and studied for decades. λ phage is also very stable and relatively easy to work with. We had a lot to learn since no one on the team had ever worked with λ phage before.

At the beginning of the summer, we received fluorescent λ phage from the Laboratoire de Physique Statistique, Ecole Normale Supérieure, Paris, France. The phage was provided by Philippe Thomen and is characterized in "Propagation of fluorescent viruses in growing plaques" by Alvarez, L. J., P. Thomen, et al. Using this phage we were able to observe the build-up of viral particles in a lytic cell.


E.coli infected with fluorescent phage. This cell is lytic: the green spots are new phage particles being constructed prior to lysis.