Week 14
From 2007.igem.org
(Difference between revisions)
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::'''10/02/07''' | ::'''10/02/07''' | ||
- | *Digestion of [http://partsregistry.org/Part: | + | *Digestion of [http://partsregistry.org/Part:pSB4A3 pSB4A3]; |
*Run on electrophoresis gel. | *Run on electrophoresis gel. | ||
*[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction. | *[http://partsregistry.org/Part:BBa_pSB4A3 pSB4A3] and [http://partsregistry.org/Part:BBa_I763033 I763033] band extraction. |
Revision as of 08:30, 22 October 2007
- 10/01/07
- Meeting: Ethical issue.
- Work on image segmentation algorithm.
- 10/02/07
- 10/03/07
- Digestions of:
- Ligations for:
Experimental Setup
- To have an higher precision for our measurements, we have decided to get, for the same field of view on the same sample of bacteria solution, either a short film segment (from which we can extract frames) and a Microsoft Excel file, which contains a ten seconds interval of values from the photomultiplier output, taken at a 100Hz frequency.
- In order to have the same focus for the microscope's oculars, the photocamera and the photomultiplier we have to calibrate our system by changing the position of both camera and photomultiplier.
- Finally, we check that the photomultiplier field of view is the same size as the microscope's one: by moving the appropiate diaphragms, we adjust the previously done settings.
- 10/04/07
- Trasformation of:
- 10/05/07
- Stock preparation (500 ul) of I763034,I763031+pSB4A3 and I763032+pSB4A3.
- Miniprep of I763034, I763031+pSB4A3 and I763032+pSB4A3.
- Control digestion of:
- Run on electrophoresis gel and I763032 (Eco/Xba and Xba/Pst1) band extraction.