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Latest revision as of 15:52, 26 October 2007

08/20/07

• I763010 and C0012 transformation.

08/21/07

• There are no colonies for I763010 and C0012. We can't amplify these biobricks.
• J04631 digestion.
• Band extraction.
• R0051+P0412 (I763010) ligation.

08/22/07

• There are no colonies for R0051+P0412 (I763010)

08/23/07

• A new R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1.2ul T4 Ligase).
• R0051, I763012 digestion with Spe/Pst1.
• P0412, J04631 digestion with Xba/Pst1.
• Run and extraction from gel.
• Transformation of R0051+P0412 (I763010) ligations.

08/24/07

• We plan new amplifications.

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