Wisconsin/Digestion
From 2007.igem.org
Endonuclease Digestion
- 10uL H2O
- 5uL DNA
- 2uL 10x buffer
- 2uL 1:10 dilution of BSA soln
- 0.5uL Enzyme 1
- 0.5uL Enzyme 2
Incubate at 37C for 2-4 hours
Use this website for restriction enzyme and this site for checking buffer compatibility.