From 2007.igem.org

The goal of our modeling work was to test our design, mainly to identify potential flaws of the system at early developmental stages.

In part II, we showed that the system can present – at least qualitatively – the desired behaviour: an exponential growth of the two populations of coexisting cellular types.

In part III, our results indicated that the system’s behavior should be reasonably robust, and provided arguments in favour of the design having a negative regulation of recombinase expression by Dap (increased robustness and tunability).

In all cases, models incorporating additional details, related to space and/or stochasticity, indicated that these phenomena should not affect the global behavior of the system. So previous conclusions, obtained using deterministic models, should remain valid despite the fact that we neglected noise- and space-related issues.

We would like to stress here that these results should be taken with great care, given the extreme simplicity of our models and the lack of data to provide information on parameter values and initial conditions. But still, globally…

…all these results corroborate our initial design.