Berkeley LBL/CompetentCellTransformation


KCM Chemical Transformation Using Chemical Competent Cells

1. Add 1-10ul ligation mixture or plasmid and 20ul KCM to 100ul cells (one aliquot). Bring total volume to 200ul using sterile water.

2. Incubate on ice for 5-30min (longer incubation gives more colonies)

3. Heat shock at 42ºC, 90sec (this step can be skipped when using ampicilin selection)

4. Plate entire solution

5. Incubate overnight in 37°C with plate upside down