Berkeley LBL/PCRcleanup


PCR Purification/ Clean Up (Using QlAquick Spin)

1. Add 5 volumes of Buffer PB or PB1for every volume of DNA

2. Transfer to QuickSpin column

3. Centrifuge for 1 min. at highest rpm to bind DNA and discard supernatant

4. Wash with 750ul Buffer PE w/ ethanol

5. Centrifuge for 1 min., discard supernatant

6. Centrifuge for an additional minute to remove excess Buffer PE

7. Transfer column to new eppendorf tube

8. Add 50ul 20% EB to elute DNA and allow to sit for 1 min.

9. Centrifuge for 1 min. and toss column.