BerkiGEM2007-ArthurConstructionFile13

From 2007.igem.org

construct ptet/rbs/wbbL/rbs/neuS in lo copy

digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest
digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest
Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy

Another version tried...

construct ptet/rbs/wbbL/rbs/neuS in lo copy

digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest
digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, BglI) 2146+1149+914, largest
Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy