BerkiGEM2007-ArthurConstructionFile13
From 2007.igem.org
construct ptet/rbs/wbbL/rbs/neuS in lo copy digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, AlwNI) 2146+1510+553, largest Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy
Another version tried...
construct ptet/rbs/wbbL/rbs/neuS in lo copy digest I716101 low copy guy (EcoRI, BamHI), 3150+910, largest digest I716008 ptet/rbs/wbbL/rbs/neuS (EcoRI, BamHI, BglI) 2146+1149+914, largest Ligate them prod I716012 ptet/rbs/wbbL/rbs/neuS in lo copy