From 2007.igem.org

8   Forward primer.   
cgacgAGATCTgctaacacgtaaactccac

9   Reverse to get the RBS, and an ATG (what they did)
gcgtgCTCGAGttaGGATCCcattgcttttcctt

10  Reverse to get without an RBS (unsure if this will work)
gcataCTCGAGttaGGATCCgccattgaatacctgtcca

13  Forward get RFP w/o start codon
cgacgAGATCTgcttcctccgaagacgttatc

14  Reverse get RFP
gcataCTCGAGttaGGATCCttattaagcaccggtggag



pcr 7/3

Template for all: I716004
For each, digest PCR prod with BglII/XhoI and ligate into pBca9145-1145 #4 (BglII/XhoI)


(I716013) (yfbE/rbs/ATG)    ay008/9      349bp
(I716014) (yfbE solo)       ay008/10     333bp
(I716015) (RFP without ATG) ay011/12     709bp