From 2007.igem.org

I716013 (pBca9145-yfbE-rbs-ATG)

ay008/9 on I716004        349bp  (BglII, XhoI)
into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
prod I716013
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ay008  cgacgAGATCTgctaacacgtaaactccac
ay009  gcgtgCTCGAGttaGGATCCcattgcttttcctt