From 2007.igem.org

make (yfbE-rbs-ATG) + (RFP_no_ATG) from pcr prod

PCR product (RFP_no_ATG), digested BglII/XhoI
Digest I716013 (yfbE-rbs-ATF) BamHI/XhoI (2387+9), L
Ligate, get I716016 (yfbE-rbs-RFP_jp)



or for rfp step, (I716015)  2063+693 get small.