From 2007.igem.org

composite <yfbE promoter><rbs><RFP>
[digest] I716014 (yfbE solo)                 (EcoRI/BamHI, 2063+ 317, S)
[digest] pBca1100-Bca1109                    (EcoRI/BamHI, 2927+1253, L)
[ligate] pBca9145-yfbE_pro-rbs-RFP (I716017)