BerkiGEM2007-AustinConstructionFile21
From 2007.igem.org
Alpha:
PCR CA1150/CA1099R on 9145-I716070 (1727 Bp)
Digest (Bsa1/Bgl2) (1686/Small, Larger) and ligate with beta portion.
Beta:
PCR CA1099F/G00101 on Part 9145-I716080 (1567 bp)
Digest (Bsa1/BamH1) (1441/small, Larger) and ligate with alpha portion.
Product is 9145-I716071