BerkiGEM2007-AustinConstructionFile23
From 2007.igem.org
PCR ca1099F/AD0005R on 9145-I716053 -HbA alpha unmutated- (1445 bp)
PCR AD0006F/ca1099R on 9145-I716053 –HbA alpha unmutated- (1577 bp)
Digest both with BsaI/DpnI, and Ligate together Product is 9145-I716073