From 2007.igem.org

Alpha:
PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)
Digest (Bsa1/Bgl2) (2111/Small, Larger) and ligate with beta portion.

Beta:

PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1) (1447/small, Larger) and ligate with alpha portion.


Product is 9145-I716074 (best yield)