BerkiGEM2007-AustinConstructionFile37

From 2007.igem.org

PCR AD0010F/AD0010R on I716054 (462 bp)
Digest PCR product (EcoR1/Nco1/DpnI, 446/11/5, Largest)

Digest petDUET (EcoR1/Nco1, 5377/43, Larger)

Ligation product is I716087

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