From 2007.igem.org

Pre-biobricking modifications to I716091.
Quickchange AD0013F/R to get rid of EcoR1 site.
Quickchange AD0014/F/R to get rid of BglII site.
Quickchange AD0015F/R to get rid of XhoI site.

PCR AD0016F/R on modified I716091.
Digest Bgl2/Xho1 and insert into 9145 plasmid.
Product is I716354