From 2007.igem.org

Biobrickificreation of I716110,111 (RBS Library + T7 RNA Polymerase: ATG, GTG)
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Digest pBca1122-Bca9235			(EcoRI/BamHI, ????+??, S)
Sub into pBca9145-I716103-T7rnap-A	(EcoRI/BglII, 4721+9, L)
And into pBca9145-I716104-T7rnap-G	(EcoRI/BglII, 4721+9, L)
Products are respectively:
pBca9145-I716110-RBS+T7rnap-A
pBca9145-I716111-RBS+T7rnap-G
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No Oligos Needed