BerkiGEM2007-KristinConstructionFile7

From 2007.igem.org

Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from CFT073

Digest pBca1101-I716051		        (EcoR1/BamH1, 2904+1065, S)
Sub into pBca9145-I71652		(EcoR1/Bg1ll, 3326+9, L)
Product is pBca9145-I716160

Retrieved from "http://2007.igem.org/wiki/index.php/BerkiGEM2007-KristinConstructionFile7"