From 2007.igem.org

[rbs][ATG][lox][term][lox][BamHI]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
using Bca1090 rbs site

---

Digest pBca9145-I716214 (BamHI/AlwnI/BglI, 1195+596+553bp, Large)
Digest pBca9145-I716210 (BglII/BglI, 1794+923bp, Large)
Product is pBca9145-I716218.

Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large)
Digest pBca9145-I716210 (BglII/BglI, 1794+923bp, Large)
Product is pBca9145-I716219.