From 2007.igem.org

[rbs][ATG][lox][term][lox][barnase]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
using Bca1090 rbs site

---

Digest pBca9145-I716214 (BamHI/AlwnI/BglI, 1195+596+553bp, Large)
Digest pBca9145-I716211 (BglII/BglI, 1488+923bp, Large)
Product is pBca9145-I716220.

Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large)
Digest pBca9145-I716211 (BglII/BglI, 1488+923bp, Large)
Product is pBca9145-I716221.