From 2007.igem.org

[rbs][ATG][lox][term][lox][BglII]
2 Different terminators (1124 and 1092 - will probably use 1092 in the end)
using Bca1090 rbs site

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Digest pBca9145-I716214 (BamHI/AlwnI/BglI, 1195+596+553bp, Large)
Digest pBca9145-Bca1147 (BglII/BglI, 1824+923bp, Large)
Product is pBca9145-I716222.

Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large)
Digest pBca9145-Bca1147 (BglII/BglI, 1824+923bp, Large)
Product is pBca9145-I716223.