From 2007.igem.org

Biobricking of ceaB without start codon

PCR sil02F and sil02R on colE2 colicin region (1758bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716209

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sil02F Forward BglII biobrick of ceaB ccaaaAGATCTagcggtggcgatggacgcgg
sil02R Reverse XholI/BamHI biobrick of ceaB gctagCTCGAGttaGGATCCttacttaccccgatgaatatc