BerkiGEM2007-SamanthaConstructionFile5

From 2007.igem.org

Biobricking of BamHI endonuclease without start codon


PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L)
Product is pBca9145-I716210


sil04F Forward BglII biobrick of BamHI
ccaaaAGATCTgaagtagaaaaagagtttattactga
sil04R Reverse XholI/BamHI biobrick of BamHI
gctagCTCGAGttaGGATCCctatttgttttcaactttatc