BerkiGEM2007-SamanthaConstructionFile9
From 2007.igem.org
[rbs][Cre]
rbs library - want a weak rbs actually
3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)
ATG Cre variant
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716224.
GTG Cre variant
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716225.
TTG Cre variants
Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716226.