From 2007.igem.org

GCTTCCCGCTCGTTCCTAACGATTCGCAGGCATATGAAGCATTGCTCGTACTCGGTCCACTGTCGCACAGAGAATTGGGATCTAAATGTCGGGTTACATCGTAATCCCGCCTAGCTGCTCGATCAAGGTGAACAGATCCTTGACTTGCAAGACCCGTGCCGCAAACGCTCTATTCAGCCAACGCAGGGAAGACATATCAACTTGAAAGCGGGCGATCGCTGCATCCGGGCTTCTATTAGCGAGATGGGTTTCGGTTGCCTGGGCATCCGCGTCTACTTTCCGCGGCGGCGATAAGCGTGGCGGCGCTAACGGCGCGCGTCTGGCATTAGCGCCTCAGCGCGACTGGGATGTCAACGCCGTTGCGGCTCGCGTTCTGCCGGTATTAGAAGAGATCCAGAAAACGACGAATAAAGCCTCGCTGGCCGATATTATTGTGCTGGCGGGCGTGGTCGGTATCGAGCAGGCGGCCGCTGCTGCGGGTGTCAGCATCAGCGTACCTTTTGCGCCGGGCCGGGTGGATGCGCGTCAGGATCAGACCGACATCGAGATGTTCTCGCTGCTTGAACCGATTGCTGATGGATTCCGTAACTATCGTGCGCGTCTGGATGTGTCAACGACCGAATCGCTGTTGATTGATAAAGCGCAGCAGTTAACGTTGACCGCGCCGGAAATGACGGTACTGGTTGGCGGGATGCGTGTGCTGGGAACCAACTTTGACGGCAGCCAGAACGGTGTCTTTACCGACAGACCGGGCGTGCTCAGCACTGACTTCTTCGCTAATCTGCTGGATATGCGTTACGAGTGGAAGCCCACCGACGACGCTAATGAGCTGTTCGAAGGCCGGGATCGTCTGACTGGCGAGGTAAAATACACGGCGACCCGCGCCGATCTGGTGTTTGGTTCCAACTCCGTACTGCGCGCGCTGGCGGAAGTTTACGCGTGTAGCGATGCGCACGAGAAGTTTGTGAAGGACTTCGTCGCGGCATGGGTGAAAGTGATGAACCTGGACCGTTTCGATCTGCAATAAGGATCCTAACTCGAGCTGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTCGGCGCGCCA

This has been reverse complemented from the source .SEQ to allow comparison with the target plasmid.