From 2007.igem.org

Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1145 for the HPI, katG open reading frame from Salmonella typhimurium LT2

 
PCR VU03F.katG /VU06R.katG on HPI, katG	(1316 bp, gp = A)
PCR VU05F.katG/VU04R.katG on HPI, katG		(924 bp, gp = B)
 ---------------------------------------------------
PCR VU03F/VU04R on A+B				(2212 bp, BglII/XhoI)
Digest pBca9145-Bca1144 (Clone # 5)		(BglII/XhoI, 2063+910, L)
Product is pBca9145-VU2 
 ---------------------------------------------------
VU03F.katG (External oligo) 	ccaaaAGATCTatgagcacgaccgacgataccc
VU04R.katG (External oligo) 	gctagCTCGAGttaGGATCCttattgcagatcgaaacggtcc
VU05F.katG (Internal oligo) 	gccgaaagaagaCctgatctggcag
VU06R.katG (Internal oligo) 	ctgccagatcagGtcttctttcggc