Bologna University/Ligation protocol

From 2007.igem.org

(final volume 20 μl)
1. Mix :
- 8 μl of insert DNA
- 4 μl of vector plasmid
- 4 μl of Buffer 5X
- 1 μl of T4 Ligase
- 3 μl of mQ water
2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.


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