Bologna University/Ligation protocol
From 2007.igem.org
- (final volume 20 μl)
- 1. Mix :
- - 8 μl of insert DNA
- - 4 μl of vector plasmid
- - 4 μl of Buffer 5X
- - 1 μl of T4 Ligase
- - 3 μl of mQ water
- 2. Incubate at 4 °C overnigth or at 15 °C for 3 hr.
- 1. Mix :
