Bologna University/Ligation reaction
From 2007.igem.org
- (final volume 20 μl)
- 1. Mix :
- - 8μl of insert DNA
- - 4μl of vector plasmid
- - 4μl of Buffer 5x [ ]
- - 1μl of T4 Ligase
- - 3μl of ultrapure water
- 2. Incubate at 4 °C overnigth or at 15 °C for 3hr.
- 1. Mix :
