Bologna University/Procedure
From 2007.igem.org
- 1. [http://partsregistry.org/Help:IGEM_07_Parts_Kit Biobricks amplification]
- 2. Digestion reaction
- 3. Run on agarose 1% gel
- 4. DNA extraction from gel (QIAquick Gel Extraction kit – Ca No 28704)
- 5. Ligation reaction
- 6. Transformation
- 7. Miniprep (QIAprep Spin Miniprep kit - Ca No 27104 Qiagen)
- 8. Stock preparation in 40% glycerol and digestion for control
