Bologna University/Transformation Buffer

From 2007.igem.org

(always made fresh)
1. To prepare 100 ml dissolve in ultrapure water:
-15mM CaCl2 0.2205 g
-250mM KCl 1.864 g
-10mM Pipes 0.302 g
2. Adjust pH with KOH to 6.7.
3. Add 55mM MnCl2 (0.89 g of MnCl2x2H2O).
4. Filter with 0.22 μm filter.


Back