From 2007.igem.org

In vivo test I

Ca2+ stock:        100mM

Ca2+  [µg/ml]	0	0.1	1.0	10
IPTG [M]	1	1	1	1
Amp [µg/ml]	50	50	50	50

With this concentration of Ca2+, Amp and IPTG were following plates pour.

3 plates IPTG

3 plates IPTG + AMP

3 plates IPTG + Amp + Ca2+ (0.1)

3 plates IPTG + Amp + Ca2+ (1.0)

3 plates IPTG + Amp + Ca2+ (10)

3 plates IPTG + Ca2+ (0.1)

3 plates IPTG + Ca2+ (1.0)

3 plates IPTG + Ca2+ (10)

500 cells/100µl were plated in each plate.

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