Freiburg07/boxes

From 2007.igem.org

Contents

Puffer und Antibiotika Box

Ladepuffer 6X hell-dunkel 1:1
Ladepuffer 6x Hell
Amp 100mg
Cm 25mg
Kan 50mg

iGem Parts Box

I15009 Klon 1,2,3 phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
I15008 Klon 1,2,3 heme oxygenase (ho1) from Synechocystis
I13453 Klon 1,2,3 Pbad promoter
B0034 Klon 1,2,3 RBS (Elowitz 1999) -- defines RBS efficiency

Lactamase Box

Plasmide:
dFR320dp_b1_wz_b2
dFR320p_b1_wz_b2
pFR320_b1_wz_b2

pAR310dp_b1_wz_b2
pAR310d_b1_wz_A2
pAR310dp_b2_wz_b1
pAR310d_b1_wz_A2
pAR300d_wz_b1_b2

Regulator Konstrukte Box

dFRdp_b1_calmo2,2_b2 Klon 1,2,3
dFRdp_b1_calmo4,4_b2 Klon 1,2,3
dFRdp_b1_calmo6,6_b2 Klon 1,2

YCG (Calmo)
FHY1

Teile Box

Calm.Ins. 2Gly
Calm.Ins. 4Gly
Calm.Ins. 6Gly
Ins.Dhfr1
Ins.Dhfr2A
dFR320dp
Insert 261
pFR320db_b1_wz_b2
pFR320d_b1_wz_b2

Dhfr Box

b1_wz_Dhfr2A
b1_wz_Dhfr2 Klon 2,3

dFR320d_b1_wz_dhfr2A
pAr3ßßd
pFR320d_wz_dhfr2a Klon 1,2