From 2007.igem.org

Contents 1 Wed, 25th July to Thu, 03th August 2007 2 Wed, 10th July to Fri, 20th July 2007 3 Mon, 25th June to Thu, 05th July 2007 4 Wed, 20th June to Thu, 28th June 2007

Wed, 25th July to Thu, 03th August 2007

we cloned the 320d blac1-wz-dhfr plasmid once again and repeated the PCR for the dhfr1 peace to create the plasmid again
the rest is listed beneath labnotes 3

Wed, 10th July to Fri, 20th July 2007

After ligating the dhfr1 fragment into the dhfr2 including vector and making several digests we came to the conclusion that something was wrong with our plasmids. sequencing the construct affirmed this

Mon, 25th June to Thu, 05th July 2007

we gained the dhfr1 fragment by using PCR. we used an inproper plasmid for the first PCR so we had to repeat it several times, using another plasmid

Wed, 20th June to Thu, 28th June 2007

Planning a new Plasmid containing splitted Dhfr Fragments instead of the lactamase
we cutted the 320d blac1-wz-blac2 plasmid (used as vector) and a 300d dhfr2 plasmid (insert) with HindIII and SpeI, ligated them and transformed into XL-1 blue E.coli cells to get a 320d blac1-wz-dhfr2 plasmid

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