From 2007.igem.org

Plasmids obtained from others

Ca2+ sensor: ß-lactamase

Plasmid containing the lactamase part 1 fragment: pAR310d-blac1-winzipA2

Plasmid containing the lactamase part 1 fragment with periplasmatic signal sequence: pAR310dp-blac1-winzipA2

Plasmid containing the lactamase part 2 fragment: pAR300d-winzipB1-blac2

Plasmid containing the lactamase part 2 fragment with periplasmatic signal sequence: pAR300dp-winzipB1-blac2

Ca2+ sensor: DHFR

Plasmid containing the dhfr1 fragment: pAR200d-GAS41reg1-dhfr1

Plasmid containing the dhfr2 fragment: pAR300d-AF10winDelV41D-dhfr2A

Light sensor: Phytochrom A with CFP and YFP

Plasmid containing PhyA1: D153ah-phyA_1-406

Plasmid containing FHY1: pGADT7ah-FHY1

Plasmid containing CFP: pAR200-cJunCFP

Plasmid containing YFP: pAR200-cFos-YFP

Plasmid containing YFP: pAR200-YFP-Dumy

Sequence of Calmodulin: Cam_yc6E_2

New plasmids (engineered by iGEM-Team-Members)

Plasmid wih both lactamase fragments: pFR320d-blac1-winzipB1-blac2
Plasmid wih both lactamase fragments and periplasmatic signal sequence: pFR320p_b1_WinzipB1_b2

Ca2+ sensor: ß-lactamase

Plasmids with calmodulin between the lactamase fragments:

2 amino acids for linker: pFR320p_b1_2calmo2_b2
4 amino acids for linker: pFR320p_b1_4calmo4_b2
6 amino acids for linker: pFR320p_b1_6calmo6_b2

Ca2+ sensor: DHFR

Plasmids with calmodulin between the dhfr fragments:

2 amino acids for linker: dFR320d-dhfr1-2calmo2-dhfr2A
4 amino acids for linker: dFR320d-dhfr1-4calmo4-dhfr2A
6 amino acids for linker: dFR320d-dhfr1-6calmo6-dhfr2A

Light sensor: Phytochrom A with CFP and YFP

pAR200-Fhy1Big-YFP
pAR200-Fhy1Small-YFP
pAR200-PhyA-His-CFP
pAR200-YFP-Fhy1Big
pAR200-YFP-Fhy1Small

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