From 2007.igem.org

Contents 1 Primers for specific ends of the dhfr1-fragment 2 Primers for calmodulin-construct with different linkerlengths 3 Primers for FHY1 4 Primers for PhyA 5 Primer for sequencing

Primers for specific ends of the dhfr1-fragment

pr-Kpn1-dhfr1: 5' TAATTAGGTA CCCAATTCCG GTTGTTC 3'

pf-Sph-h-dhfr1: 5' TATCGACGCA TGCACCATCA CCATCACCAT ATGGTTCGAC CATTGAAC 3'

Primers for calmodulin-construct with different linkerlengths

forward primers:

pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3'
pf-calmo-KpnI-linker3: 5' TATCGACGGT ACCGGCGGTG GCGAGCAGAT TGCAGAGTTC AAAG 3'
pf-calmo-KpnI-linker4: 5' TATCGACGGT ACCGGCGGTG GCGGTGAGCA GATTGCAGAG TTCAAAG 3'
pf-calmo-KpnI-linker6: 5' TATCGACGGT ACCGGCGGTG GCTCTGGTGG CGAGCAGATT GCAGAGTTCA AAG 3'
pf-calmo-KpnI-linker9: 5' TATCGACGGT ACCGGCGGTG GCGGTTCTGG CGGTGGCGGT GAGCAGATTG CAGAGTTCAA AG 3'

reverse primers:

pr-calmo-SpeI-linker2: 5' AACGATCACT AGTACCTCCC TTTGCTGTCA TCATTTGTAC A 3'
pr-calmo-SpeI-linker3: 5' AACGATCACT AGTACCGCCA CCGCCCTTTG CTGTCATCAT TTGTACA 3'
pr-calmo-SpeI-linker4: 5' AACGATCACT AGTGCCACCG CCTCCCTTTG CTGTCATCAT TTGTACA 3'
pr-calmo-SpeI-linker6: 5' AACGATCACT AGTACCGCCA CCAGAGCCAC CCTTTGCTGT CATCATTTGT ACA 3'
pr-calmo-SpeI-linker9: 5' AACGATCACT AGTACCGCCA CCGCCAGAAC CGCCACCGCC CTTTGCTGTC ATCATTTGTA CA 3'

Primers for FHY1

FP_FHY1-Big_NheI: 5' TATCGACGCT AGCGATTACA TCTATGGGAC TCAGA 3'

FP_FHY1_NheI: 5' TATCGACGCT AGCAGTAGTA ATGCTGCTAA GTTTGT 3'

RP_FHY1_Asc1: 5' AACGATCGGC GCGCCCAGCA TTAGCGTTGA GAAGTAT 3'

Primers for PhyA

FP_PhyA_EcoRI-XbaI_SD: 5' TATCGACGAA TTCGCGGCCG CTTCTAGAAA GAGGAGAAAT TAACTATGTC AGGCTCTAGG CCGAC 3'

RP_PhyA_SpeI_Linker: 5' AACGATCACT AGTTCCGCCG TGATGGTGAT GGTGGCCTCC GTTATCGAGT TCCACCTCCT TA 3'

Primer for sequencing

pQE RP: 5' GTTCTGAGGTCATTACTGG 3'

pQE FP: 5' CGGATAACAATTTCACACAG 3'

sf-calm-80: 5' GGAGCTGAATGAAGCC 3'

sr-dhfr3: 5' AAACTTATACTTGATGC 3'

BACK