From 2007.igem.org

PCR HNX-F and HNX-R on H-Nox (567bp, BglII/XhoI) Sub into pBca1144 ( BglII/XhoI, 2054+910 L) Product is pBca1144-H-NOX

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HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG

HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA