Journal Club 09

From 2007.igem.org

14:30pm, Apr 22nd Sun, Biotechnology building

We have invited Dr Liu Shufan from UCSD to join our Journal Club.

Contents

Idea discussion

Ultraviolet light induced Red/Green flip in E.coli

Presented by Lou Chunbo.

(using E.coli's SOS response) 

--P_RM--=cI=>--=lacI=>--=GFP=>--<=RFP=--P_L--
            LexA  |
              |   | double repress
              -   -
             -=====-=cI_ind=>--


Substitute of GFP/RFP (other reporter enzyme): lacZ luc..

An abstraction of this system:

  1. A cell with multi-stable states.
  2. Each stable state has a memory element.
  3. When triggered by outside excitation, the cell goes to another state (decided by memory element).

Can the input be POPS?

大家讨论

李拓：希望我们的设计像一个black box，具有比较通用的特性，可以与别的模块连接使用。

杨一帆：同上，最好能换一种诱导方式，而不是UV。

刘书摹：这个idea有意思，如果能做成功是一个很有新意的工作。但是设计细节需要仔细斟酌。可能出现的问题是：1，UV照射对细菌的状态有全局影响，细菌的反应可能出现混乱；2，LacI是一个很强的抑制子，光靠分裂稀释可能不足以去除其作用；3，CI不是一个好的转录激活因子，不一定能用；4，两个反向转录而相互抑制的启动子，可以考虑使用phage186。

倪鸣：使用UV照射的一个原因是LexA的转录调控很清楚，会给我们的设计带来很多方便。

Summary of Tianjin Workshop

Tianjin Univ iGEM Workshop

Drew Endy's session

Presented by Yang Yifan.

Inspiration we get from Drew Endy's story of electric circuit: The naturally evolved bio-systems are not for understanding of human beings. We should design our own bio-systems.

POPS is a key idea from iGEM.

About project idea:

  1. It must be COOL.
  2. There should be progressive milestones. In the last 2 years, no more than 10% of the teams had biologically feasible products when they went to the Jumboree.

Christina Smolke's session

Presented by Liu Xili.

用RNA实现基因调控。利用适配子与RNA自催化酶（RNA自催化酶是一段具有斧头形二级结构的RNA，能催化自身降解）组合成调控元件。适配子结合了特定的小分子后，二级结构改变，进而影响RNA自催化酶的二级结构，触发其降解，由此可调控mRNA的水平。利用类似原理还可以控制RNAi过程。

Team logistics

Journal Club 10/11

Job assignments

See this page.

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