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Contents 1 Experiments 2 Analysis 3 Inference 4 Discussions

Experiments

Microscopy:

• Open loop trial - 50 ng/ml aTc
• Full range induction of pL CFP for equivalence. NOT final because OD was >0.1 (~0.4-1.0)

Analysis

Microscopy:

• Open loop trial - 50 ng/ml aTc

1. For both cases of AI (grown at 50 ng/mL and ODs 0.2/2), make Scatter plots of CFP vs LuxR.YFP for all 6 concentrations of IPTG.
2. A combined plot of Mean and S.D of CFP and LuxR.Y having points from OD 0.2 in one colour and OD 2 in the other colour.

Scatter Plots Green : AI OD 2 Blue : AI OD 0.2

File:Scatter openloop J21.jpg Mean and Standard Error Plots Red : AI OD 2 Blue : AI OD 0.2 for CFP , YFP, RFP Linear and Log space Inference aTc being an antibiotic may have adverse effects on the cell at high concentrations such as 100 ng/ml. Growth rate is reduced and cells are killed. These factors may in turn affect the expression of the fluorescence gene which may lead to a drop in mean fluorescence intensity at high concentration levels. Discussions