From 2007.igem.org

Preparing Antibiotics:

The following is the protocol for preparing Kanamycin and Ampicillin from their stock compunds:

1. Add less than 5ml dH20 to a large volume tube (blue caps) eg. 4ml.
2. Add 500ug Amp stock ->100ug/ml or, 50ug Kan stock -> 10ug/ml.
3. Add water till the final volume is made up to 5ml.
4. Filter sterilize (remember the syringe screws onto the filter to be secured, not pushed in.
5. With a P1000 pipette, extract 1ml into 5 epitubes.
6. Store @ -20C.