From 2007.igem.org

<return to plans page>

1. Retransform c1 inverter (afternoon + overnight)
2. Liquid culture (overnight)
3. Isolate plasmid (mini prep kit not working - to big? - best way to isolate?) (half day)
4. Confirm Isolation and part using XbaI/PstI digest (half day)
   • If feeling optimistic digest OmpR promoter simultaneously with SpeI/PstI and prepare to gel purify fragments of OmpR promoter (vector) and the inverter(insert)
5. If positive ligate (overnight)
6. Transform and liquid culture several colonies to confirm (1 afternoon + overnight + 15min + overnight)
7. Miniprep and digest to confirm and glycerol stocks. (1 day)

Next need to ligate to RBS-ComA-Ter construct.