Melbourne/GvpL site dirrected primers
From 2007.igem.org
Primer sequences: Primer Name Primer Sequence (5' to 3')
| g213a | 5'-aggatatgagctggctgcaagaaaaagcatttcatcatc-3' |
| g213a_antisense | 5'-gatgatgaaatgctttttcttgcagccagctcatatcct-3' |
| g318a | 5'-ttataaaggtgaagaaagtctgcaagcagctattgagattaacaaag-3' |
| g318a_antisense | 5'-ctttgttaatctcaatagctgcttgcagactttcttcacctttataa-3' |
| g351a | 5'-attgagattaacaaagaaaagatagaaaattcactgacgctgcttc-3' |
| g351a_antisense | 5'-gaagcagcgtcagtgaattttctatcttttctttgttaatctcaat-3' |
| g696a | 5'-ttcttctcccgtctctgcaaattactaagttcgtaaacg-3' |
| g696a_antisense | 5'-cgtttacgaacttagtaatttgcagagacgggagaagaa-3' |
Oligonucleotide information: Tm and CG content verified from http://mbcf.dfci.harvard.edu/docs/oligocalc.html
| Primer Name | Length (nt.) | Tm | Duplex Energy at 68°C | Energy Cost of Mismatches | GC content | Tm ver | OD=1 | bp before | bp after |
|---|---|---|---|---|---|---|---|---|---|
| g213a | 39 | 78.45°C | -44.89 kcal/mole | 6.5% | 41% | 81 | 2216nM | 19 | 19 |
| g213a_antisense | 39 | 78.45°C | -42.10 kcal/mole | 7.8% | 41% | 81 | 2216nM | ||
| g318a | 47 | 78.97°C | -46.02 kcal/mole | 6.3% | 30% | 80 | 1754nM | 25 | 21 |
| g318a_antisense | 47 | 78.97°C | -42.40 kcal/mole | 7.7% | 30% | 80 | 1754nM | ||
| g351a | 46 | 78.02°C | -43.40 kcal/mole | 5.3% | 33% | 80 | 1820nM | 26 | 19 |
| g351a_antisense | 46 | 78.02°C | -40.27 kcal/mole | 7.4% | 33% | 80 | 1820nM | ||
| g696a | 39 | 78.45°C | -42.46 kcal/mole | 5.4% | 41% | 81 | 2496nM | 19 | 19 |
| g696a_antisense | 39 | 78.45°C | -43.97 kcal/mole | 6.8% | 41% | 81 | 2496nM |
