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Contents 1 Primers used for PCR stitching of Blue photoreceptor 2 Oligo 15 3 Sequencing primer for Blue photosensor construct (spans stitch) 4 PsrfA Primers

Primers used for PCR stitching of Blue photoreceptor

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-- Oligo 1 --

  Sequence Name: BL_Con1_s
  Sequence: Cagtcggtgcggacatcgttgcttgtaagcaaagcttatac
  Number of Bases: 41
  Tm:68

-- Oligo 2 --

  Sequence Name: BL_Con1_as
  Sequence: GTATAAGCTTTGCTTACAAGCAACGATGTCCGCACCGACTG
  Number of Bases: 41
  Tm:68

-- Oligo 3 --

  Sequence Name: BL_Con2_s
  Sequence: TcggtgcggacatcgctgCTTGTAAGCAAAGCTTATACCTTTG
  Number of Bases: 43
  Tm:69

-- Oligo 4 --

  Sequence Name: BL_Con2_as
  Sequence: CAAAGGTATAAGCTTTGCTTACAAGCAGCGATGTCCGCACCGA
  Number of Bases: 43
  Tm:69

-- Oligo 5 --

  Sequence Name: BL_Con3_s
  Sequence: GGTGCGGACATCGCTGGAGGTAAGCAAAGCTTATACCTTTGAG
  Number of Bases: 43
  Tm:70

-- Oligo 6 --

  Sequence Name: BL_Con3_as
  Sequence: CTCAAAGGTATAAGCTTTGCTTACCTCCAGCGATGTCCGCACC
  Number of Bases: 43
  Tm:70

-- Oligo 7 --

  Sequence Name: BL_Con4_s
  Sequence: GCGGACATCGCTGGAGGACAGCAAAGCTTATACCTTTGAGGT
  Number of Bases: 42
  Tm:70

-- Oligo 8 --

  Sequence Name: BL_Con4_as
  Sequence: ACCTCAAAGGTATAAGCTTTGCTGTCCTCCAGCGATGTCCGC
  Number of Bases: 42
  Tm:70

-- Oligo 9 --

  Sequence Name: BL_Con5_s
  Sequence: GACATCGCTGGAGGACGCCAAAGCTTATACCTTTGAGGTTACTC
  Number of Bases: 44
  Tm:70

-- Oligo 10 --

  Sequence Name: BL_Con5_as
  Sequence: GAGTAACCTCAAAGGTATAAGCTTTGGCGTCCTCCAGCGATGTC
  Number of Bases: 44
  Tm:70

-- Oligo 11 --

  Sequence Name: BL_Con6_s
  Sequence: gacgcagagcaggcacaaaagGTGATATTTTTAGATAAGCATGAAGTT
  Number of Bases: 48
  Tm:67

-- Oligo 12 --

  Sequence Name: BL_Con6_as
  Sequence: AACTTCATGCTTATCTAAAAATATCACCTTTTGTGCCTGCTCTGCGTC
  Number of Bases: 48
  Tm:67

-- Oligo 13 --

  Sequence Name: BL_Con7_s
  Sequence: GGCAGAGGAGATCAACACGGAAGTTGGACCGGACTGGAATT
  Number of Bases: 41
  Tm:70

-- Oligo 14 --

  Sequence Name: BL_Con7_as
  Sequence: AATTCCAGTCCGGTCCAACTTCCGTGTTGATCTCCTCTGCC
  Number of Bases: 41
  Tm:70

Oligo 15

  Sequence Name: BL_FP1_s
  Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT
  Number of Bases: 48
  Tm:74
  *(fwd primer: prefix-SopII)

Sequencing primer for Blue photosensor construct (spans stitch)

-- Oligo 16 --

  Sequence Name: BL_seq_v1
  Sequence: ACCTCGATGTCGAGCTTGAG
  Number of Bases: 20
  Tm:54

PsrfA Primers

-- Oligo 17 --

  Sequence Name: BL_psrfA_fwd
  Sequence: AGCTAGGaattcgcggccgcttctagATGGTGGGACTTACGACCCTCT
  Number of Bases: 48
  Tm:74

-- Oligo 18 --

  Sequence Name: BL_psrfA_rev
  Sequence: GGGATTGGTAAGCTGGTTTCAGtactagtagcggccgctgcagagctag
  Number of Bases: 49
  Tm:74