Melbourne/meeting 14 September 2007

From 2007.igem.org

Contents

5:30 Action points from last meeting:

  • Jan: Plan for blue light on wiki by 17th August- Done but needs more work: couldn’t get anyone else to implement it based on this!

5:35 Status Reports: (5 minutes each including any discussion)

  • Pat : Red light receptor progress/design/plan- needs?
    • M30109: Must construct from scratch plan to be produced by Pat.
    • This is then drives RBS-ComP-term
    • Also required is ConstitutiveProm-RBS-ComA-term


  • Jan & Alisa: Blue light receptor design/plan progress - needs?
    • Primers ComA and Com P all in
    • Rapid Ligase 5min purchased
    • ComP and SopII to be conbined at variouse points by using 4 primer stiching:
      • Comp P forward
      • SopII reverse
      • ComP-SoPII overlap sense
      • ComP-SopII overlap antisense
    • Note that there are 7 different stiching pairs that result in combination at different points.
    • The product is then combined with RBS
    • Then transcription terminator
    • The constitutive promotor to create protein generator part.
    • Also Required is reporter construct PsfPromoter-RBS-LacZ-term
  • Phil: Gas vesicles progress/design/plan - needs?
    • Primers for part creation in
    • No Progress , round 4 of mutagenesis plated awaits culture and diggest(4 plates).
    • Buoyancy experiment needs repeating
    • Other parts required from kit->glycerol stocks: B034 (P1-3O) , B0015 (P1-1I or P3-3O), J61034 (P4-8F or 8H)
    • Pat to provide these.

5.55 New Agenda Items:

  • Phil: Administrative Timetable: (5 Weeks to go)


  • Chris: Next years Comp Plan/preparations - no progress


  • Next meeting: <b/> 21th September (Friday) 5.30PM.

6:15 End

Igem Administrative Timetable:

1 October Final team rosters due.
1 October Jamboree attendance fees due $100USD per team member.
26 October Project and wiki & part documentation frozen, DNA received USA.
3-4 November iGEM Competition Jamboree, MIT, USA.


.


  • re prism :Edmund optics:VIS60 Schott veril linear variable interference filter SGD$1600 25mm wide 60mm long linear 400nm-700nm (contact sheet)

N47-279 eqilateral prism 35mm long 35mm face SGD$162


Documentation wiki status:

    • Priority should be on plans and experimental report analysis and conclusions-----Alisa .
    • If only Pat & Alisa doing lab work not much documentation needed.
    • Equipement:
      • MissingLas3000 method (in lab notebook)
      • Missing DNA measurement protocol (eppendorf)
    • Reagents pages incomplete:
      • Some location pictures not finished or uploaded.
      • Some newer reagents not entered.
    • Secondary reagents pages not made:
      • LB, Plates, TAE , NZY+ Broth
    • Protocol part:
      • No connections from Protocols to reagents.
      • Ligation protocol missing
    • Lab notebook:
      • Format uses too many lines to see connections between days.
      • Latter part missing links
      • Can table of contents be restricted to date level?
    • Experimental reports:
      • Lacks discussion/conclusion
      • Ligation experiment missing
      • Lattest few gells not marked up and entered.
    • Project overview link broken
    • Background:
      • GFP missing ,
      • Red photo sensor to GFP test harness missing.
    • Plan: Only Gas vesicles plan entered.


IndividualDesired time committment
Pat About 4h/wk Monday afternoon or Tuesday morning
Alisa About 4h/wk - Thursday afternoon
Jan About 10h/pw - flexible weekday mornings
Phil About 18h/pw - Thursday morning, Friday, Saturday or Sunday,