Melbourne/meeting 17 August 2007
From 2007.igem.org
Contents |
5:50 Action points from last meeting:
- Alisa& Pat: Wiki "Parts_used status" up to date? -(outstanfding from 19/7/07)-Done
- Pat & Alisa: Plan for red light receptor on wiki -(Lac Z reporter outstanding )
- Alisa, Paul & Jan: Review blue light theory - done
- Jan:Theory for blue light on wiki-done
- Jan:ComP fusion to be ordered.- 31 August shipping
- Jan:Plan for blue light on wiki by 17th August- Done (needs more details- coundn't implement this)
- Jan:<b/>Order red and blue filters.-Phill to pick up
- <b>Paul: Status of #BBa-M30109 & #BBa-V1012 lost in transit?- Shipped
- Craig: Alkaline lysis technique for miniprep to be documented-link to open wetware?
- Cheng: Daisy to produce competent cells of DB3.1 and Enz- strains.- waiting on strains still
- Chris: Gas vesicle expression experiment to be run.
Status Reports: (5 minutes each including any discussion)
- Alisa: Ligation experiments progress.
- Attempting ligation of RBS to LacZ reporter.
- Phil to culture on Sunday
- Phil & Pat to miniprep and digest on Monday
- Pat & Alisa: Red light receptor progress/design/plan- needs?
- phase 1 osmolarity test of YFP under enz
- phase 2 Red light driving YFP
- Phil to pick P2-13K colonies on sunday (Kan) and miniprep monday
- Nothing planned for this week due to academic load.
- Jan: Blue light receptor design/plan - needs?
- Nothing this week
- Phil: Gas vesicles progress/design/plan - needs?
- Plan up to basic biobrick complete,
- Second mutagenesis under way. Problem with first apears to be PCR fixed on second by increesing seg1 to 2 min, seg 2 to 1min and elongation to 15minutes
- Chris to start expression experiment cultures
- Phil to set up bouyancy test experiment with cultures on saturday and photograph throughout week
- Craig to provide protocol for protein extraction
- Need to check expression of gas vesicles Chris to perform.
- Plan for support parts/test harness required.
- Lac promotor and antibiotic death plasmids required.
- Kasia: Documentation progress vs last report/ new problems/ needs.-No report
New Agenda Items:
- Phil: Administrative Timetable: (10 Weeks to go)
- Next meeting: <b/> 21th August (Friday) 5.30PM.
7:00 End
Igem Administrative Timetable:
| 1 September | Final team rosters due. |
| 1 September | Jamboree attendance fees due $100USD per team
member. |
| 26 October | Project and part documentation due DNA received USA. |
| 26 October | BioBrick Part DNA received by the Registry. |
| 3-4 November | iGEM Competition Jamboree, MIT, USA. |
.
Documentation wiki status:
- Priority should be on plans and experimental report analysis and conclusions-----Alisa .
- If only Pat & Alisa doing lab work not much documentation needed.
- Equipement:
- MissingLas3000 method (in lab notebook)
- Missing DNA measurement protocol (eppendorf)
- Reagents pages incomplete:
- Some location pictures not finished or uploaded.
- Some newer reagents not entered.
- Secondary reagents pages not made:
- LB, Plates, TAE , NZY+ Broth
- Protocol part:
- No connections from Protocols to reagents.
- Ligation protocol missing
- Lab notebook:
- Format uses too many lines to see connections between days.
- Latter part missing links
- Can table of contents be restricted to date level?
- Experimental reports:
- Lacks discussion/conclusion
- Ligation experiment missing
- Lattest few gells not marked up and entered.
- Project overview link broken
- Background:
- GFP missing ,
- Red photo sensor to GFP test harness missing.
- Plan: Only Gas vesicles plan entered.
| Individual | Desired time committment |
|---|---|
| Pat | About 4h/wk Monday afternoon or Tuesday morning |
| Alisa | About 4h/wk - Thursday afternoon |
| Jan | About 10h/pw - flexible weekday mornings |
| Phil | About 18h/pw - Thursday morning, Friday, Saturday or Sunday, |
