Melbourne/prefixsuffix primers

From 2007.igem.org

  • Melting temperatures of the overlaps to the gene sequence range from 82degC to 90 degC
  • GC content ranges from 42% to 61%
  • The primers not only allow all ORFs to be extracted but can also be used to sequence the whole polycystronic gene.
Name Forward primer Sequence: 5’-…..-3’
NL26-F GAATTCGCGGCCGCTTCTAGATAGAAGGATTTACGCGC
GvpA-F GAATTCGCGGCCGCTTCTAGATGAGTATTCAAAAAAGTACAG
GvpP-F GAATTCGCGGCCGCTTCTAGATGTCAACAACTGATAACAATATG
NL29-F GAATTCGCGGCCGCTTCTAGGAAAGAAGCTGGAGAAAAC
GvpQ-F GAATTCGCGGCCGCTTCTAGATGGATAAAAAAGATGTGGAG
GvpB-F GAATTCGCGGCCGCTTCTAGATGTCTATTCAAAAAAGTACTAATAG
GvpR-FGAATTCGCGGCCGCTTCTAGATGGAAATTAAAAAAATTATGCAAGC
GvpN-FGAATTCGCGGCCGCTTCTAGATGACCGTCTTAACAGAC
GvpF-FGAATTCGCGGCCGCTTCTAGATGAGTGAAACAAACGAAACAGG
GvpG-FGAATTCGCGGCCGCTTCTAGATGCTTCACAAATTAGTAACCGC
GvpL-FGAATTCGCGGCCGCTTCTAGATGGGAGAATTACTGTATTTATAC
GvpS-FGAATTCGCGGCCGCTTCTAGATGTCTCTTAAACAATCCATGG
GvpK-FGAATTCGCGGCCGCTTCTAGATGCAACCGGTCAGCC
GvpJ-FGAATTCGCGGCCGCTTCTAGATGGCAGTCGAACATAATATG
GvpT-FGAATTCGCGGCCGCTTCTAGATGGCAACTGAAACAAAATTAG
GvpU-FGAATTCGCGGCCGCTTCTAGATGAGTACAGGCCCTTC
Name Reverse primer Sequence: 5’-…..-3’
NL26-R AAGGCTGCAGCGGCCGCTACTAGTACTGCAGCAAGCAGAAGC
GvpA-R AAGGCTGCAGCGGCCGCTACTAGTATTAAAAGCTAAGCCCTGC
GvpP-R AAGGCTGCAGCGGCCGCTACTAGTATTACTTCTTCGCGTCCATG
GvpQ-R AAGGCTGCAGCGGCCGCTACTAGTATTATGATCCCATTGTTTTTAAATC
GvpB-R AAGGCTGCAGCGGCCGCTACTAGTATTAAATACTAAAACGCGGCTG
GvpR-RAAGGCTGCAGCGGCCGCTACTAGTATTATGCTTCAATGCCAATGG
GvpN-RAAGGCTGCAGCGGCCGCTACTAGTATTACTTTTCTTCAGTGTCTATATTC
GvpF-RAAGGCTGCAGCGGCCGCTACTAGTATTATTTCTCTTCTACTTTTAGGC
GvpG-RAAGGCTGCAGCGGCCGCTACTAGTACTAGGATTCCTCATTTCTTTTTTGTG
GvpL-RAAGGCTGCAGCGGCCGCTACTAGTATTACGCAAAGCTCGAGAAATG
GvpS-RAAGGCTGCAGCGGCCGCTACTAGTATCAGGATCCAAGTGGATTC
GvpK-RAAGGCTGCAGCGGCCGCTACTAGTATTAAAGCAGGCTGCCTAGC
GvpJ-RAAGGCTGCAGCGGCCGCTACTAGTATTAACGTTTCGTTTCTATTTTTTCTTC
GvpT-RAAGGCTGCAGCGGCCGCTACTAGTATTAGGCTGTTGTATCGTCTTC
GvpU-RAAGGCTGCAGCGGCCGCTACTAGTATTATGAACTCTTTTTACTCGTTG