From 2007.igem.org

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Contents 1 Physical data 2 Primer design and maintenance 3 Problems 4 Lab Location 5 Manufacturer:

Physical data

• Description: Forward primer for backbone vector
• Uses: Sequencing inserts into the backbone vector/colony PCR/PCR of insert

Primer design and maintenance

• Primers were designed identical to the sequence in the [http://partsregistry.org/Part:BBa_G00100 VF2 page] on the Registry.
• Primers were ordered and purchased from Sigma
• We keep a working concentration of 10uM and a stock solution of 1mM.

Problems

• Problems have been reported for these primers in which the primers are able to bind to sequences from the double terminator sequence, [http://partsregistry.org/Part:BBa_B0010 B0010], a part that we commonly use.

• See [http://partsregistry.org/Problems_with_PCR_using_VR/VF2 here] for more details.

Lab Location

• Location: Fz1 Amp freezer west side of central lab
• Permision to use required?No

Manufacturer:

• Manufacturer:Sigma
• Manufacturers