From 2007.igem.org

[http://www.krackeler.com/products/fid/2753 Unit Definition of Restriction Enzymes]

• One unit is defined as the amount of enzyme required to hydrolyze 1µg of λ DNA to completion in 60 minutes in a total reaction volume of 50 µl, under optimal assay conditions (salt concentration, pH, temperature).
• Some enzymes which do not, or rarely, cleave λ DNA. DNA are tested using another substrate; for example, enzymes such as Not I or Sfi I are tested with adenovirus DNA, and Fok I is tested using pBR322.

[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/setting_up_reaction.asp Setting Up a Restriction Endonuclease Reaction]

[http://www.promega.com/guides/re_guide/chaptwo/2_1.htm Standard Restriction Enzyme Reactions]

[http://www.neb.com/nebecomm/products/faqcategory1.asp FAQs for Restriction Endonucleases]

[http://www.ndsu.edu/instruct/mcclean/plsc731/dna/dna5.htm Restriction Modification System]

[http://www.everythingbio.com/glos/definition.php?word=star+activity Definition of star activity]

• AatII
• AccI
• AciI
• AseI
• BamHI
• BanII
• BbsI
• BbvI
• BglII
• BsaBI
• BspEI
• EcoRI
• EcoRV
• HindIII
• MboI
• MspI
• NcoI
• NdeI
• NheI
• NotI
• PacI
• PstI
• RsaI
• SacI
• SalI
• SmaI
• SpeI
• SwaI
• XbaI
• XhoI
• XmaI
• XmnI

Back