From 2007.igem.org

• Attendants: Chih-Hsien anf sakula (domain-based protein designer)
• Title: Fusion construct for signaling transduction to regualte gene expression
• Motivation
  • No read-to-use promoter for glucose-sensing in E.coli
  • Most sensory protein have similar responsive domain (e.g. Histidine Kinase domain) and varied in signal sensing domain
• Strategy:  Fuse glucose-sensing domain of membrance sensory protein to a known signal relay
• Reference
  • [http://www.nature.com/nature/journal/v438/n7067/abs/nature04405.html Engineering Escherichia coli to see light, 2005 Nature]
  • File:Osmotic shift.png (osmotic shift, K+)
  • light sensing domain PCB from cyanobacteria is fused with EnvZ
• Material:
  • comparison between EnvZ and RcsC
    • File:EnvZ.png (EnvZ, osmotic sensory protein)
    • File:EnvZ domain.png (EnvZ domains)
    • File:RcsC.png (RcsC, glucose sensory protein)
    • File:RcsC domain.png (RcsC domains)
  • ompR binding site 
    • <a href="http://partsregistry.org/Part:BBa_R0082">Part:BBa_R0082</a> (15P, plate 1)
    • <a href="http://partsregistry.org/Part:BBa_R0083">Part:BBa_R0083</a> (17H, plate 1)
    • <a href="http://partsregistry.org/Part:BBa_R0084">Part:BBa_R0084</a> (11H, plate 1)